Introduction to Image Analysis 2025 cover image
9:30 AM — 11:00 AM
Working with ImageJ
  • Fiji and ImageJ
  • ImageJ interface
  • Basic concepts and common tasks
  • Opening, visualizing and saving an image
Luís Marques picture
Luís Marques
11:00 AM — 11:20 AM
Coffee Break
11:20 AM — 12:30 PM
Exercises
Ana Beatriz Barbosa picture
Ana Beatriz Barbosa
Diogo Coutinho picture
Diogo Coutinho
Patrícia Rodrigues picture
Patrícia Rodrigues
Gabriel G. Martins picture
Gabriel G. Martins
12:30 PM — 2:00 PM
Lunch
2:00 PM — 3:00 PM
Multi-dimensional images
  • 5D images: x, y, z, channels and time
  • Basic operations
Pedro Campinho picture
Pedro Campinho
3:00 PM — 3:15 PM
Coffee Break
3:15 PM — 4:30 PM
Exercises
Ana Beatriz Barbosa picture
Ana Beatriz Barbosa
Diogo Coutinho picture
Diogo Coutinho
Patrícia Rodrigues picture
Patrícia Rodrigues
Gabriel G. Martins picture
Gabriel G. Martins
9:30 AM — 11:00 AM
Quantitative bioimage analysis
  • Images as a source of data
  • Regions of interest
  • Line profile
  • ROI Manager
Hugo Botelho picture
Hugo Botelho
11:00 AM — 11:20 AM
Coffee Break
11:20 AM — 12:30 PM
Exercises
Ana Beatriz Barbosa picture
Ana Beatriz Barbosa
Diogo Coutinho picture
Diogo Coutinho
Patrícia Rodrigues picture
Patrícia Rodrigues
Gabriel G. Martins picture
Gabriel G. Martins
12:30 PM — 2:00 PM
Lunch
2:00 PM — 3:00 PM
Spatial measurements in ImageJ
  • Pixel spatial calibration
  • Setting a scale bar
  • Measurement types
Telmo Pereira picture
Telmo Pereira
3:00 PM — 3:15 PM
Coffee Break
3:15 PM — 4:30 PM
Exercises
Ana Beatriz Barbosa picture
Ana Beatriz Barbosa
Diogo Coutinho picture
Diogo Coutinho
Patrícia Rodrigues picture
Patrícia Rodrigues
Gabriel G. Martins picture
Gabriel G. Martins
9:30 AM — 11:00 AM
Image pre-processing and segmentation
  • Basics of linear filtering
  • Thresholding
  • Basics of Binary filtering
  • Object counting
Manuel Tanqueiro picture
Manuel Tanqueiro
11:00 AM — 11:20 AM
Coffee Break
11:20 AM — 12:30 PM
Exercises
Ana Beatriz Barbosa picture
Ana Beatriz Barbosa
Diogo Coutinho picture
Diogo Coutinho
Patrícia Rodrigues picture
Patrícia Rodrigues
Gabriel G. Martins picture
Gabriel G. Martins
12:30 PM — 2:00 PM
Lunch
2:00 PM — 3:00 PM
Advanced Segmentation
  • Labkit
  • Stardist
Anna Pezzarossa picture
Anna Pezzarossa
3:00 PM — 3:15 PM
Coffee Break
3:15 PM — 4:15 PM
Introduction to automation
  • Using the macro recorder
  • Introduction to batch processing
  • Best practices in reporting bioimage quantification
Anna Pezzarossa picture
Anna Pezzarossa
4:15 PM — 5:00 PM
Exercises and Wrap-Up
Ana Beatriz Barbosa picture
Ana Beatriz Barbosa
Diogo Coutinho picture
Diogo Coutinho
Patrícia Rodrigues picture
Patrícia Rodrigues
Gabriel G. Martins picture
Gabriel G. Martins