21st May
Introduction to Image Analysis
22nd May
Introduction to QuPath
23rd May
Workflows
2:00 PM
— 2:15 PM
Presentation of PPBI
2:15 PM
— 2:55 PM
What is an Image?
2:55 PM
— 3:35 PM
Types of images, colormaps and stain vectors
3:35 PM
— 3:50 PM
Coffee Break
3:50 PM
— 4:30 PM
Concepts of thresholding, ML and DL
4:30 PM
— 5:00 PM
QuPath in the world of pathology
9:30 AM
— 10:15 AM
How to handle images on QuPath?
- Image properties
- Create a project
10:15 AM
— 11:15 AM
Tools - Annotations
- Types of ROIs available and how to use them
- Hierarchy
- Properties and Classes
- Calculating features
- Exercises
11:15 AM
— 11:30 AM
Coffee Break
11:30 AM
— 12:30 PM
Tools - Detections
- Cell detection
- Properties, Measurements and tips
- Positive Cell Detection
- Exercises
12:30 PM
— 2:00 PM
Lunch Break
2:00 PM
— 2:45 PM
Stain Vectors
- Setting a stain vector
- Estimating a stain vector
2:45 PM
— 3:45 PM
Pixel Classification
- Tissue detection
- Create and measure objects
- Exercises
3:45 PM
— 4:00 PM
Coffee Break
4:00 PM
— 4:30 PM
Object Classification
- Training an object classifier (machine learning)
4:30 PM
— 5:00 PM
Density Maps
- Creating a density map
- Finding hotspots
- Creating annotations based on density
9:30 AM
— 10:30 AM
Multiplexed analysis
- Visualization of multiple channels
- Cell Detection
- Creating a cell classifier
- Exercises
10:30 AM
— 11:30 AM
Object Classification
- Training an object classifier (machine learning)
- Training images
- Composite classifiers
- Improving training
- Heatmaps
11:30 AM
— 11:45 AM
Coffee Break
11:45 AM
— 12:15 PM
Pixel Classification
- Training a pixel classifier (machine learning)
- Creating objects based on classifier
12:15 PM
— 1:00 PM
Introduction to groovy scripting
- Automated scripts
- Extensions - Stardist and Cellpose (deep learning)
1:00 PM
— 2:00 PM
Lunch Break
2:00 PM
— 4:00 PM
Working on your images
- wrap-up
- call4help